The SF6 gas toxicity biological test method aims to simulate the composition of atmospheric gases, specifically by mixing 79% nitrogen (N2) and 21% oxygen (O2), which represents the typical air composition. In this test, mice are continuously exposed to a controlled environment containing SF6 gas for a period of 24 hours. After this exposure, the animals are observed for an additional 72 hours in normal air conditions. The primary objective is to monitor the mice for any signs of abnormal behavior, physical distress, or mortality. Based on these observations, researchers can determine whether the SF6 gas sample is toxic to living organisms.
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